This study is designed to survey and analyze the data requirements for the Sakaerat Biosphere Reserve's database system. An open questionnaire was used with 423 people in online form and in meetings, including students, university students, teachers, researchers, and ordinary people. The answers from the questionnaire were categorized by keywords and analyzed as mean percentage and average, then the answers were selected based on Pugh matrix analysis (PMA). The results showed the following demands for the spatial database is area size; the biodiversity database is species lists of each living thing in area and taxonomy; the meteorology database is daily weather forecasts; the research database is expertise, research topic, and researcher names; the science camp database is mission, activity details, and route to the area. Requirements for presentation formats are images and videos, whereas data files are more required for meteorology and the results of research databases. The results can be used to develop a database system for Sakaerat Biosphere Reserve and others that responds to the needs of actual users of the system.