The objectives of this research were: 1) to design and develop a 2D adventure game based on the Thai literature, Huajai Chai Noom, 2) to develop students' knowledge of the Thai literature Hua Jai Chai Noom through a 2D adventure game, and 3) to assess the level of knowledge of students in Thai literature Hua Jai Chai Noom through a 2D adventure game. The 2D game development process involved analyzing the Hua Jai Chai Noom storyline to design game elements, creating 2D graphics, and utilizing Aseprite as the primary software for developing characters, scenes, buttons, and animations. Construct2 was employed as the main platform for overall game development. The sample group consisted of 40 Grade 10 students who participated in the game testing. The researcher collected gameplay scores from each round and recorded the number of rounds required to complete the game. The data were analyzed using the mean (), standard deviation (S.D.), and repeated measures ANOVA.
       The research findings indicate that the 2D Adventure Game Based on the Thai Literature Huajai Chai Noom was developed completely according to the concept and content specified. The results of the development of knowledge found that the average score increased in each round. In the first round, the score was 7.03, in the second round, it was 10.93, and in the third round, it was 13.75, and statistical analysis of the repeated measures ANOVA test, it was found that the players' knowledge increased statistically at the 0.01 level (F = 98.50, p < 0.01), which could be examined in the 1st, 2nd and 3rd rounds of play in conclusion, playing the adventure game repeatedly in each round had an effect on the change in students' knowledge, making the groups of students have a more similar understanding of the content, and the results of the players using an average of only 1.85 rounds of play show that most players can quickly understand the content and question-answering mechanisms within the game, learning from their mistakes in the first round and applying the knowledge.